African Journal of Paediatric Surgery

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 12  |  Issue : 4  |  Page : 247--250

A corrosive oesophageal burn model in rats: Double-lumen central venous catheter usage


Vedat Bakan1, Harun Çiralik2, Seyfi Kartal3 
1 Department of Pediatric Surgery, Faculty of Medicine, Turgut Ozal University, Ankara 06510, Turkey
2 Department of Pathology, Faculty of Medicine, Sütçü İmam University, Kahramanmaraş 46100, Turkey
3 Department of Anesthesiology and Reanimation, Faculty of Medicine, Turgut Ozal University, Ankara 06510, Turkey

Correspondence Address:
Dr. Vedat Bakan
Department of Pediatric Surgery, Faculty of Medicine, Turgut Ozal University, Ankara 06510
Turkey

Background: We aimed to create a new and less invasive experimental corrosive oesophageal burn model using a catheter without a gastric puncture (gastrotomy). Materials and Methods: We conducted the study with two groups composed of 8 male rats. The experimental oesophageal burn was established by the application of 10% sodium hydroxide to the distal oesophagus under a pressure of 20 cmH 2 O, via 5-F double-lumen central venous catheter without a gastrotomy. The control group was given 0.9% sodium chloride. All rats were killed 24 h after administration of NaOH or 0.9% NaCl. Histologic damage to oesophageal tissue was scored by a single pathologist blind to groups. Results: The rats in the control group were observed to have no pathological changes. Corrosive oesophagitis (tissue congestion, oedema, inflammation, ulcer and necrosis) was observed in rats exposed to NaOH. Conclusion: We believe that an experimental corrosive oesophageal burn can safely be created under same hydrostatic pressure without a gastric puncture using this model.


How to cite this article:
Bakan V, Çiralik H, Kartal S. A corrosive oesophageal burn model in rats: Double-lumen central venous catheter usage.Afr J Paediatr Surg 2015;12:247-250


How to cite this URL:
Bakan V, Çiralik H, Kartal S. A corrosive oesophageal burn model in rats: Double-lumen central venous catheter usage. Afr J Paediatr Surg [serial online] 2015 [cited 2020 Sep 19 ];12:247-250
Available from: http://www.afrjpaedsurg.org/article.asp?issn=0189-6725;year=2015;volume=12;issue=4;spage=247;epage=250;aulast=Bakan;type=0