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ORIGINAL ARTICLE
Year : 2014  |  Volume : 11  |  Issue : 3  |  Page : 233-237

Tumour necrosis factor-alpha gene polymorphisms in Iranian patients with biliary atresia


1 Gentic Department, Center of Excellence for Biodiversity, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
2 Pediatric Department, Pediatric Health Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
3 Department of Pediatrics, Pediatric Health Research Center, Liver & Gastrointestinal Disease Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

Correspondence Address:
Prof. Mandana Rafeey
Department of Pediatrics, Pediatric Health Research Center, Children Hospital, Sheshgelan, P. O. Box: 5136735886, Tabriz
Iran
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Source of Support: This project was financially supported by Paediatric Health Research Centre, Tabriz University of Medical Sciences., Conflict of Interest: None


DOI: 10.4103/0189-6725.137332

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Background: Biliary atresia (BA) is a progressive inflammatory destructive process of the bile ducts. This study evaluated the relationship between single-nucleotide polymorphisms in the promoter region of tumour necrosis factor-alpha (TNF-α) gene and bilaiary atresia. Materials and Methods: Genomic deoxyribonucleic acid from 16 patients with established diagnosis of BA and 36 patients with INC was obtained. The genotypes of TNF-α-1031 (T/C) and TNF-α-308 (G/A) were determined using the restriction fragment length polymorphism-polymerase chain reaction and the results were analysis with proper statistic software. Results: The frequencies of T/T, T/C in TNF-α-1031 and G/G, G/A in TNF-α-308 were as same as control group. Moreover, we have same deduction for allele frequency and haplotypes analysis (T allele: 84.37%; G allele: 87.5%) in BA patients (T allele: 80.56%; G allele: 86.11%) in controls. In all cases variants of polymorphism did not affect the severity or incidence of BA disease. Conclusion: although no significant associations were found between BA and control groups, it seems meaningful that since the nature of BA is multi factorial. Next step will be considering a new target such as downstream modulation of the TNF-α pathway or other cytokines and chemokines which act directly/indirectly.


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